Sphingomyelin synthase is absent from endosomes
نویسندگان
چکیده
منابع مشابه
Sphingomyelin synthase is absent from endosomes.
Both the Golgi and the endosomes have recently been proposed as the main site of SM-synthase, the enzyme responsible for sphingomyelin (SM) biosynthesis. To settle this confusion, we studied the subcellular distribution of SM-synthase in human liver-derived HepG2 and baby hamster kidney BHK-21 cells. To discriminate between Golgi and endosomes we made use of 3,3-diaminobenzidine (DAB) cytochemi...
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BACKGROUND It has been proposed that plasma sphingomyelin (SM) plays a very important role in plasma lipoprotein metabolism and atherosclerosis. Sphingomyelin synthase (SMS) is the last enzyme for SM de novo biosynthesis. Two SMS genes, SMS1 and SMS2, have been cloned and characterized. METHODS AND RESULTS To evaluate the in vivo role of SMS2 in SM metabolism, we prepared SMS2 knockout (KO) a...
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Japanese encephalitis virus (JEV) is a mosquito-borne RNA virus which infects target cells via the envelope protein JEV-E. However, its cellular targets are largely unknown. To investigate the role of sphingomyelin (SM) in JEV infection, we utilized SM-deficient immortalized mouse embryonic fibroblasts (tMEF) established from SM synthase 1 (SMS1)/SMS2 double knockout mice. SMS deficiency signif...
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Sphingolipids and the derived gangliosides have critical functions in spermatogenesis, thus mutations in genes involved in sphingolipid biogenesis are often associated with male infertility. We have generated a transgenic mouse line carrying an insertion in the sphingomyelin synthase gene Sms1, the enzyme which generates sphingomyelin species in the Golgi apparatus. We describe the spermatogene...
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Tissue injury in inflammation involves the release of several cytokines that activate sphingomyelinases and generate ceramide. In the lung, the impaired metabolism of surfactant phosphatidylcholine (PC) accompanies this acute and chronic injury. These effects are long-lived and extend beyond the time frame over which tumor necrosis factor (TNF)-alpha and interleukin-1beta are elevated. In this ...
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 1997
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.110.6.781